Leukotriene C4 EIA Kit

Background

Leukotriene C4 (LTC4) is a potent mediator of immediate hypersensitivity, bronchoconstriction, smooth muscle contraction, increased vascular permeability, and epithelial mucous secretion. This competitive ELISA assay is based on the competition between the LTC4 present in your sample and the LTC4-enzyme conjugate for a limited number of antibody binding sites.

Assay Principle

This is an ELISA  for the quantitative analysis of LTC4 levels in biological fluid.  This test kit operates on the basis of competition between the enzyme conjugate and the LTC4 in the sample for a limited number of binding sites.

First, the sample or standard solution is added to the microplate.  Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour.  During the incubation, competition for binding sites is taking place.  The plate is then washed, removing all the unbound material.  The bound enzyme conjugate is detected by the addition of substrate which generates an optimal color after 30 minutes. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 450nm or 650nm.  The extent of color development is inversely proportional to the amount of LTC4 in the sample or standard.  For example, the absence of LTC4 in the sample will result in a bright blue color, whereas the presence of LTC4 will result in decreased or no color development.

If using urine as a sample it is recommended to run our

Creatinine Assay kit (CR01)

in conjunction with your samples since urinary creatinine levels may be used to normalize the rate of excretion of other analytes.

Cross Reactivity

Leukotriene C4                    100%
Leukotriene D4                 14.04%
Leukotriene E4                   7.80%
Leukotriene B4                 <0.01%
Prostaglandin A2              <0.01%
Prostaglandin B2              <0.01%
Prostaglandin D2              <0.01%
Prostaglandin E2              <0.01%
Prostaglandin F2α             <0.01%
6-keto-Prostaglandin F1α  <0.01%
5-HETE                            <0.01%
12-HETE                          <0.01%
15-HETE                          <0.01%
6-trans-Leukotriene B4     <0.01%
20-OH-Leukotriene B4      <0.01%
Thromboxane B3              <0.01%